second harmonic generator bbo crystal Search Results


second harmonic generator shg  (CH Instruments)
90
CH Instruments second harmonic generator shg
Second Harmonic Generator Shg, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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second harmonic generation signal shg  (Oxford Instruments)
99
Oxford Instruments second harmonic generation signal shg
Second Harmonic Generation Signal Shg, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/second+harmonic+generator+bbo+crystal/Imaris/pmc05071700__EJI___46___1415___s001-2-22-32
Average 99 stars, based on 1 article reviews
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monolayer mos2  (Photonics Inc)
86
Photonics Inc monolayer mos2
Monolayer Mos2, supplied by Photonics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/second+harmonic+generator+bbo+crystal/monolayer+mos2/arxiv__2307__11839-407-19-22
Average 86 stars, based on 1 article reviews
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rabbit polyclonal active caspase 3  (Danaher Inc)
99
Danaher Inc rabbit polyclonal active caspase 3
(a) Schematic of experiments to study the effects of altering boundary constraints in fibrous environments (i.e., stretch, blue symbols, or release of prestrain, red symbols). (b) Both stretch (4%→9%) and release (9%→4%) resulted in changes in nuclear aspect ratio revealing strain transfer from the scaffold to bovine annulus fibrosus cells (statistical significance was determine using an unpaired two-tailed t-test between static and stretch or release, n = 512 4% Static, 747 4%→9%, 845 9% Static, and 706 9%→4% cells per group from 3 scaffolds per group). Monitoring spontaneous calcium signaling (c, d) before and after altering the boundary constraints revealed a significant increase in the percentage of cells responding (e, statistical significance was determined using a paired two-tailed t-test, where scaffolds were paired by donor and experiment, n = 6 scaffolds per group, 25 cells per scaffold. A single cell’s trace is shown for a 10-minute test following strain release with this cell outlined with a white dashed line. Release of prestrain (9%→4%) triggered apoptosis as assayed 24 hours after actuation, while stretch (4%→9%) did not, as evidenced by active <t>caspase</t> <t>3</t> staining (f-j; statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds per group from 3 donors). (k) Traction force microscopy showed (l) blocking the ROCK pathway with either Y27632 or Fasudil decreased AF cell contractility (statistical significance was determined using Kruskal-Wallis testing with Dunn’s multiple comparisons post hoc, n = 16 control and n = 14 Y27632 and fasudil) and (m-q) attenuated the apoptotic response triggered by loss of prestrain (statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds for control and 4 for Y27632 and Fasudil per group where scaffolds represent independent samples). Bars denote mean and standard deviation.
Rabbit Polyclonal Active Caspase 3, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/second+harmonic+generator+bbo+crystal/Rabbit+Polyclonal+Anti-JAK2+(phospho+Y1007)+antibody/pmc06899202-157-19-24
Average 99 stars, based on 1 article reviews
rabbit polyclonal active caspase 3 - by Bioz Stars, 2026-11
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wavelength meter ws6  (HighFinesse GmbH)
90
HighFinesse GmbH wavelength meter ws6
(a) Schematic of experiments to study the effects of altering boundary constraints in fibrous environments (i.e., stretch, blue symbols, or release of prestrain, red symbols). (b) Both stretch (4%→9%) and release (9%→4%) resulted in changes in nuclear aspect ratio revealing strain transfer from the scaffold to bovine annulus fibrosus cells (statistical significance was determine using an unpaired two-tailed t-test between static and stretch or release, n = 512 4% Static, 747 4%→9%, 845 9% Static, and 706 9%→4% cells per group from 3 scaffolds per group). Monitoring spontaneous calcium signaling (c, d) before and after altering the boundary constraints revealed a significant increase in the percentage of cells responding (e, statistical significance was determined using a paired two-tailed t-test, where scaffolds were paired by donor and experiment, n = 6 scaffolds per group, 25 cells per scaffold. A single cell’s trace is shown for a 10-minute test following strain release with this cell outlined with a white dashed line. Release of prestrain (9%→4%) triggered apoptosis as assayed 24 hours after actuation, while stretch (4%→9%) did not, as evidenced by active <t>caspase</t> <t>3</t> staining (f-j; statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds per group from 3 donors). (k) Traction force microscopy showed (l) blocking the ROCK pathway with either Y27632 or Fasudil decreased AF cell contractility (statistical significance was determined using Kruskal-Wallis testing with Dunn’s multiple comparisons post hoc, n = 16 control and n = 14 Y27632 and fasudil) and (m-q) attenuated the apoptotic response triggered by loss of prestrain (statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds for control and 4 for Y27632 and Fasudil per group where scaffolds represent independent samples). Bars denote mean and standard deviation.
Wavelength Meter Ws6, supplied by HighFinesse GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/second+harmonic+generator+bbo+crystal/ws6+600+wavelength+meter/pm35568551-69-12-15
Average 90 stars, based on 1 article reviews
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ktiopo4  (Laurell Technologies Corporation)
86
Laurell Technologies Corporation ktiopo4
(a) Schematic of experiments to study the effects of altering boundary constraints in fibrous environments (i.e., stretch, blue symbols, or release of prestrain, red symbols). (b) Both stretch (4%→9%) and release (9%→4%) resulted in changes in nuclear aspect ratio revealing strain transfer from the scaffold to bovine annulus fibrosus cells (statistical significance was determine using an unpaired two-tailed t-test between static and stretch or release, n = 512 4% Static, 747 4%→9%, 845 9% Static, and 706 9%→4% cells per group from 3 scaffolds per group). Monitoring spontaneous calcium signaling (c, d) before and after altering the boundary constraints revealed a significant increase in the percentage of cells responding (e, statistical significance was determined using a paired two-tailed t-test, where scaffolds were paired by donor and experiment, n = 6 scaffolds per group, 25 cells per scaffold. A single cell’s trace is shown for a 10-minute test following strain release with this cell outlined with a white dashed line. Release of prestrain (9%→4%) triggered apoptosis as assayed 24 hours after actuation, while stretch (4%→9%) did not, as evidenced by active <t>caspase</t> <t>3</t> staining (f-j; statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds per group from 3 donors). (k) Traction force microscopy showed (l) blocking the ROCK pathway with either Y27632 or Fasudil decreased AF cell contractility (statistical significance was determined using Kruskal-Wallis testing with Dunn’s multiple comparisons post hoc, n = 16 control and n = 14 Y27632 and fasudil) and (m-q) attenuated the apoptotic response triggered by loss of prestrain (statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds for control and 4 for Y27632 and Fasudil per group where scaffolds represent independent samples). Bars denote mean and standard deviation.
Ktiopo4, supplied by Laurell Technologies Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/second+harmonic+generator+bbo+crystal/ktiopo4/10__1364_slash_oe__24__030225-12-27-13
Average 86 stars, based on 1 article reviews
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broken symmetry iii v semiconductor fano metasurfaces  (Photonics Inc)
86
Photonics Inc broken symmetry iii v semiconductor fano metasurfaces
(a) Schematic of experiments to study the effects of altering boundary constraints in fibrous environments (i.e., stretch, blue symbols, or release of prestrain, red symbols). (b) Both stretch (4%→9%) and release (9%→4%) resulted in changes in nuclear aspect ratio revealing strain transfer from the scaffold to bovine annulus fibrosus cells (statistical significance was determine using an unpaired two-tailed t-test between static and stretch or release, n = 512 4% Static, 747 4%→9%, 845 9% Static, and 706 9%→4% cells per group from 3 scaffolds per group). Monitoring spontaneous calcium signaling (c, d) before and after altering the boundary constraints revealed a significant increase in the percentage of cells responding (e, statistical significance was determined using a paired two-tailed t-test, where scaffolds were paired by donor and experiment, n = 6 scaffolds per group, 25 cells per scaffold. A single cell’s trace is shown for a 10-minute test following strain release with this cell outlined with a white dashed line. Release of prestrain (9%→4%) triggered apoptosis as assayed 24 hours after actuation, while stretch (4%→9%) did not, as evidenced by active <t>caspase</t> <t>3</t> staining (f-j; statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds per group from 3 donors). (k) Traction force microscopy showed (l) blocking the ROCK pathway with either Y27632 or Fasudil decreased AF cell contractility (statistical significance was determined using Kruskal-Wallis testing with Dunn’s multiple comparisons post hoc, n = 16 control and n = 14 Y27632 and fasudil) and (m-q) attenuated the apoptotic response triggered by loss of prestrain (statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds for control and 4 for Y27632 and Fasudil per group where scaffolds represent independent samples). Bars denote mean and standard deviation.
Broken Symmetry Iii V Semiconductor Fano Metasurfaces, supplied by Photonics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/second+harmonic+generator+bbo+crystal/circuitry+iii+semiconductor+v/arxiv__2002__06594-171-20-27
Average 86 stars, based on 1 article reviews
broken symmetry iii v semiconductor fano metasurfaces - by Bioz Stars, 2026-11
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x-ray second harmonic generation  (Yabashi Industries)
90
Yabashi Industries x-ray second harmonic generation
(a) Schematic of experiments to study the effects of altering boundary constraints in fibrous environments (i.e., stretch, blue symbols, or release of prestrain, red symbols). (b) Both stretch (4%→9%) and release (9%→4%) resulted in changes in nuclear aspect ratio revealing strain transfer from the scaffold to bovine annulus fibrosus cells (statistical significance was determine using an unpaired two-tailed t-test between static and stretch or release, n = 512 4% Static, 747 4%→9%, 845 9% Static, and 706 9%→4% cells per group from 3 scaffolds per group). Monitoring spontaneous calcium signaling (c, d) before and after altering the boundary constraints revealed a significant increase in the percentage of cells responding (e, statistical significance was determined using a paired two-tailed t-test, where scaffolds were paired by donor and experiment, n = 6 scaffolds per group, 25 cells per scaffold. A single cell’s trace is shown for a 10-minute test following strain release with this cell outlined with a white dashed line. Release of prestrain (9%→4%) triggered apoptosis as assayed 24 hours after actuation, while stretch (4%→9%) did not, as evidenced by active <t>caspase</t> <t>3</t> staining (f-j; statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds per group from 3 donors). (k) Traction force microscopy showed (l) blocking the ROCK pathway with either Y27632 or Fasudil decreased AF cell contractility (statistical significance was determined using Kruskal-Wallis testing with Dunn’s multiple comparisons post hoc, n = 16 control and n = 14 Y27632 and fasudil) and (m-q) attenuated the apoptotic response triggered by loss of prestrain (statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds for control and 4 for Y27632 and Fasudil per group where scaffolds represent independent samples). Bars denote mean and standard deviation.
X Ray Second Harmonic Generation, supplied by Yabashi Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/second+harmonic+generator+bbo+crystal/x+ray+second+harmonic+generation/pm35594356-304-22-16
Average 90 stars, based on 1 article reviews
x-ray second harmonic generation - by Bioz Stars, 2026-11
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nanozoomer s210 slide scanner  (Hamamatsu)
90
Hamamatsu nanozoomer s210 slide scanner
(a) Schematic of experiments to study the effects of altering boundary constraints in fibrous environments (i.e., stretch, blue symbols, or release of prestrain, red symbols). (b) Both stretch (4%→9%) and release (9%→4%) resulted in changes in nuclear aspect ratio revealing strain transfer from the scaffold to bovine annulus fibrosus cells (statistical significance was determine using an unpaired two-tailed t-test between static and stretch or release, n = 512 4% Static, 747 4%→9%, 845 9% Static, and 706 9%→4% cells per group from 3 scaffolds per group). Monitoring spontaneous calcium signaling (c, d) before and after altering the boundary constraints revealed a significant increase in the percentage of cells responding (e, statistical significance was determined using a paired two-tailed t-test, where scaffolds were paired by donor and experiment, n = 6 scaffolds per group, 25 cells per scaffold. A single cell’s trace is shown for a 10-minute test following strain release with this cell outlined with a white dashed line. Release of prestrain (9%→4%) triggered apoptosis as assayed 24 hours after actuation, while stretch (4%→9%) did not, as evidenced by active <t>caspase</t> <t>3</t> staining (f-j; statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds per group from 3 donors). (k) Traction force microscopy showed (l) blocking the ROCK pathway with either Y27632 or Fasudil decreased AF cell contractility (statistical significance was determined using Kruskal-Wallis testing with Dunn’s multiple comparisons post hoc, n = 16 control and n = 14 Y27632 and fasudil) and (m-q) attenuated the apoptotic response triggered by loss of prestrain (statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds for control and 4 for Y27632 and Fasudil per group where scaffolds represent independent samples). Bars denote mean and standard deviation.
Nanozoomer S210 Slide Scanner, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/second+harmonic+generator+bbo+crystal/nanozoomer/pmc11829002__41467_2025_56299_MOESM7_ESM-8-15-13
Average 90 stars, based on 1 article reviews
nanozoomer s210 slide scanner - by Bioz Stars, 2026-11
90/100 stars
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harmonic generator  (Coherent Corp)
93
Coherent Corp harmonic generator
(a) Schematic of experiments to study the effects of altering boundary constraints in fibrous environments (i.e., stretch, blue symbols, or release of prestrain, red symbols). (b) Both stretch (4%→9%) and release (9%→4%) resulted in changes in nuclear aspect ratio revealing strain transfer from the scaffold to bovine annulus fibrosus cells (statistical significance was determine using an unpaired two-tailed t-test between static and stretch or release, n = 512 4% Static, 747 4%→9%, 845 9% Static, and 706 9%→4% cells per group from 3 scaffolds per group). Monitoring spontaneous calcium signaling (c, d) before and after altering the boundary constraints revealed a significant increase in the percentage of cells responding (e, statistical significance was determined using a paired two-tailed t-test, where scaffolds were paired by donor and experiment, n = 6 scaffolds per group, 25 cells per scaffold. A single cell’s trace is shown for a 10-minute test following strain release with this cell outlined with a white dashed line. Release of prestrain (9%→4%) triggered apoptosis as assayed 24 hours after actuation, while stretch (4%→9%) did not, as evidenced by active <t>caspase</t> <t>3</t> staining (f-j; statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds per group from 3 donors). (k) Traction force microscopy showed (l) blocking the ROCK pathway with either Y27632 or Fasudil decreased AF cell contractility (statistical significance was determined using Kruskal-Wallis testing with Dunn’s multiple comparisons post hoc, n = 16 control and n = 14 Y27632 and fasudil) and (m-q) attenuated the apoptotic response triggered by loss of prestrain (statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds for control and 4 for Y27632 and Fasudil per group where scaffolds represent independent samples). Bars denote mean and standard deviation.
Harmonic Generator, supplied by Coherent Corp, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/second+harmonic+generator+bbo+crystal/Harmonic+Generator+System/10__1364_slash_oe__26__013311-253-18-21
Average 93 stars, based on 1 article reviews
harmonic generator - by Bioz Stars, 2026-11
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chameleon visionii multiphoton laser  (Coherent Corp)
99
Coherent Corp chameleon visionii multiphoton laser
(a) Schematic of experiments to study the effects of altering boundary constraints in fibrous environments (i.e., stretch, blue symbols, or release of prestrain, red symbols). (b) Both stretch (4%→9%) and release (9%→4%) resulted in changes in nuclear aspect ratio revealing strain transfer from the scaffold to bovine annulus fibrosus cells (statistical significance was determine using an unpaired two-tailed t-test between static and stretch or release, n = 512 4% Static, 747 4%→9%, 845 9% Static, and 706 9%→4% cells per group from 3 scaffolds per group). Monitoring spontaneous calcium signaling (c, d) before and after altering the boundary constraints revealed a significant increase in the percentage of cells responding (e, statistical significance was determined using a paired two-tailed t-test, where scaffolds were paired by donor and experiment, n = 6 scaffolds per group, 25 cells per scaffold. A single cell’s trace is shown for a 10-minute test following strain release with this cell outlined with a white dashed line. Release of prestrain (9%→4%) triggered apoptosis as assayed 24 hours after actuation, while stretch (4%→9%) did not, as evidenced by active <t>caspase</t> <t>3</t> staining (f-j; statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds per group from 3 donors). (k) Traction force microscopy showed (l) blocking the ROCK pathway with either Y27632 or Fasudil decreased AF cell contractility (statistical significance was determined using Kruskal-Wallis testing with Dunn’s multiple comparisons post hoc, n = 16 control and n = 14 Y27632 and fasudil) and (m-q) attenuated the apoptotic response triggered by loss of prestrain (statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds for control and 4 for Y27632 and Fasudil per group where scaffolds represent independent samples). Bars denote mean and standard deviation.
Chameleon Visionii Multiphoton Laser, supplied by Coherent Corp, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/second+harmonic+generator+bbo+crystal/Chameleon/pm30776427-96-14-13
Average 99 stars, based on 1 article reviews
chameleon visionii multiphoton laser - by Bioz Stars, 2026-11
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extreme ultraviolet second harmonic generation spectroscopy  (Yabashi Industries)
90
Yabashi Industries extreme ultraviolet second harmonic generation spectroscopy
(a) Schematic of experiments to study the effects of altering boundary constraints in fibrous environments (i.e., stretch, blue symbols, or release of prestrain, red symbols). (b) Both stretch (4%→9%) and release (9%→4%) resulted in changes in nuclear aspect ratio revealing strain transfer from the scaffold to bovine annulus fibrosus cells (statistical significance was determine using an unpaired two-tailed t-test between static and stretch or release, n = 512 4% Static, 747 4%→9%, 845 9% Static, and 706 9%→4% cells per group from 3 scaffolds per group). Monitoring spontaneous calcium signaling (c, d) before and after altering the boundary constraints revealed a significant increase in the percentage of cells responding (e, statistical significance was determined using a paired two-tailed t-test, where scaffolds were paired by donor and experiment, n = 6 scaffolds per group, 25 cells per scaffold. A single cell’s trace is shown for a 10-minute test following strain release with this cell outlined with a white dashed line. Release of prestrain (9%→4%) triggered apoptosis as assayed 24 hours after actuation, while stretch (4%→9%) did not, as evidenced by active <t>caspase</t> <t>3</t> staining (f-j; statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds per group from 3 donors). (k) Traction force microscopy showed (l) blocking the ROCK pathway with either Y27632 or Fasudil decreased AF cell contractility (statistical significance was determined using Kruskal-Wallis testing with Dunn’s multiple comparisons post hoc, n = 16 control and n = 14 Y27632 and fasudil) and (m-q) attenuated the apoptotic response triggered by loss of prestrain (statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds for control and 4 for Y27632 and Fasudil per group where scaffolds represent independent samples). Bars denote mean and standard deviation.
Extreme Ultraviolet Second Harmonic Generation Spectroscopy, supplied by Yabashi Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/second+harmonic+generator+bbo+crystal/extreme+ultraviolet+second+harmonic+generation+spectroscopy/pm36729720-323-52-29
Average 90 stars, based on 1 article reviews
extreme ultraviolet second harmonic generation spectroscopy - by Bioz Stars, 2026-11
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Image Search Results


(a) Schematic of experiments to study the effects of altering boundary constraints in fibrous environments (i.e., stretch, blue symbols, or release of prestrain, red symbols). (b) Both stretch (4%→9%) and release (9%→4%) resulted in changes in nuclear aspect ratio revealing strain transfer from the scaffold to bovine annulus fibrosus cells (statistical significance was determine using an unpaired two-tailed t-test between static and stretch or release, n = 512 4% Static, 747 4%→9%, 845 9% Static, and 706 9%→4% cells per group from 3 scaffolds per group). Monitoring spontaneous calcium signaling (c, d) before and after altering the boundary constraints revealed a significant increase in the percentage of cells responding (e, statistical significance was determined using a paired two-tailed t-test, where scaffolds were paired by donor and experiment, n = 6 scaffolds per group, 25 cells per scaffold. A single cell’s trace is shown for a 10-minute test following strain release with this cell outlined with a white dashed line. Release of prestrain (9%→4%) triggered apoptosis as assayed 24 hours after actuation, while stretch (4%→9%) did not, as evidenced by active caspase 3 staining (f-j; statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds per group from 3 donors). (k) Traction force microscopy showed (l) blocking the ROCK pathway with either Y27632 or Fasudil decreased AF cell contractility (statistical significance was determined using Kruskal-Wallis testing with Dunn’s multiple comparisons post hoc, n = 16 control and n = 14 Y27632 and fasudil) and (m-q) attenuated the apoptotic response triggered by loss of prestrain (statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds for control and 4 for Y27632 and Fasudil per group where scaffolds represent independent samples). Bars denote mean and standard deviation.

Journal: Nature biomedical engineering

Article Title: Aberrant mechanosensing in injured intervertebral discs as a result of boundary-constraint disruption and residual-strain loss

doi: 10.1038/s41551-019-0458-4

Figure Lengend Snippet: (a) Schematic of experiments to study the effects of altering boundary constraints in fibrous environments (i.e., stretch, blue symbols, or release of prestrain, red symbols). (b) Both stretch (4%→9%) and release (9%→4%) resulted in changes in nuclear aspect ratio revealing strain transfer from the scaffold to bovine annulus fibrosus cells (statistical significance was determine using an unpaired two-tailed t-test between static and stretch or release, n = 512 4% Static, 747 4%→9%, 845 9% Static, and 706 9%→4% cells per group from 3 scaffolds per group). Monitoring spontaneous calcium signaling (c, d) before and after altering the boundary constraints revealed a significant increase in the percentage of cells responding (e, statistical significance was determined using a paired two-tailed t-test, where scaffolds were paired by donor and experiment, n = 6 scaffolds per group, 25 cells per scaffold. A single cell’s trace is shown for a 10-minute test following strain release with this cell outlined with a white dashed line. Release of prestrain (9%→4%) triggered apoptosis as assayed 24 hours after actuation, while stretch (4%→9%) did not, as evidenced by active caspase 3 staining (f-j; statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds per group from 3 donors). (k) Traction force microscopy showed (l) blocking the ROCK pathway with either Y27632 or Fasudil decreased AF cell contractility (statistical significance was determined using Kruskal-Wallis testing with Dunn’s multiple comparisons post hoc, n = 16 control and n = 14 Y27632 and fasudil) and (m-q) attenuated the apoptotic response triggered by loss of prestrain (statistical significance was determined using an unpaired two-tailed t-test, n = 6 scaffolds for control and 4 for Y27632 and Fasudil per group where scaffolds represent independent samples). Bars denote mean and standard deviation.

Article Snippet: Blocking was conducted with a commercial blocking reagent (Millipore product #20783) followed by primary antibody incubation overnight at 4°C (rabbit polyclonal active caspase 3, Abcam antibody ab49822 1:500; alpha smooth muscle actin, Millipore Sigma antibody a2547 1:400).

Techniques: Two Tailed Test, Staining, Microscopy, Blocking Assay, Standard Deviation